A Simple Enhancement for Gibson Isothermal Assembly Brian A Rabe and Constance Cepko bioRxiv, doi: https://doi.org/10.1101/2020.06.14.150979 【概要】 一本鎖DNA結合タンパクを付加すれば複数DNA断片でのGibson Assemblyの精度が4倍（80%!）、効率…
Sensory coding mechanisms revealed by optical tagging of physiologically defined neuronal types Donghoon Lee, Maiko Kume, Timothy E. Holy Science, 13 Dec 2019: Vol. 366, Issue 6471, pp. 1384-1389 DOI: 10.1126/science.aax8055【概要】 GCaMP-…
A highly homogeneous expansion microscopy polymer composed of tetrahedron-like monomers Ruixuan Gao, Chih-Chieh (Jay) Yu, Linyi Gao, Kiryl D Piatkevich, Rachael L Neve , Srigokul Upadhyayula & Edward S Boyden bioRxiv, posted October 22, 20…
SYNPLA: A synapse-specific method for identifying learning-induced synaptic plasticity loci Kim Dore, Yvonne Pao, Jose Soria Lopez, Sage Aronson, Huiqing Zhan, Sanchari Ghosh, Sabina Merrill, Anthony M. Zador, Roberto Malinow, Justus M. Ke…
DNA Microscopy: Optics-free Spatio-genetic Imaging by a Stand-Alone Chemical Reaction Joshua A.Weinstein, Aviv Regev, and Feng Zhang Cell, Volume 178, Issue 1, 27, Pages 229-241.e16., doi: 10.1016/j.cell.2019.05.019【概要】 DNAが任意分子の…
Prospective, brain-wide labeling of neuronal subclasses with enhancer-driven AAVs Lucas T. Graybuck, Adriana E. Sedeño-Cortés, Thuc Nghi Nguyen, Miranda Walker, Eric Szelenyi, Garreck Lenz, La’Akea Sieverts, Tae Kyung Kim, Emma Garren, Bri…
Delivering genes across the blood-brain barrier: LY6A, a novel cellular receptor for AAV-PHP.B capsids Qin Huang , Ken Y. Chan, Isabelle G. Tobey, Yujia Alina Chan,Tim Poterba, Christine L. Boutros, Alejandro B. Balazs, Richard Daneman, Jo…
Expanding the Optogenetics Toolkit by Topological Inversion of Rhodopsins Cell, 175, 4, 1131-1140.e11 Brown J, Behnam R, Coddington L, Tervo DGR, Martin K, Proskurin M, Kuleshova E, Park J, Phillips J, Bergs ACF, Gottschalk A, Dudman JT, K…
1. Alhadeff et al., Cell (2018) A Neural Circuit for the Suppression of Pain by a Competing Need State そこはかとない欧米感 2. Partridge, Front. Pharmacol. (2015) Utilizing GCaMP transgenic mice to monitor endogenous Gq/11-coupled receptor…
jGCaMP7 cultured neuron data Unpublished, Douglas Kim and Colleagues論文ではありませんが、JaneliaのGENIEがGCaMPの新シリーズを出しているようですのでご紹介。jGCaMP7。全部で4種類。 jGCaMP7s - sensitive detection of activity jGCaMP7f - more se…
The Neuronal Gene Arc Encodes a Repurposed Retrotransposon Gag Protein that Mediates Intercellular RNA Transfer Cell, 172(1-2), 275-288.e18 Pastuzyn ED, Day CE, Kearns RB, Kyrke-Smith M, Taibi AV, McCormick J, Yoder N, Belnap DM, Erlendsso…
Glyoxal as an alternative fixative to formaldehyde in immunostaining and super‐resolution microscopy The EMBO Journal (2017) e201695709, DOI 10.15252/embj.201695709 Richter KN, Revelo NH, Seitz KJ, Helm MS, Sarkar D, Saleeb RS, D'Este E, E…
A non-canonical Notch complex regulates adherens junctions and vascular barrier function William J. Polacheck, Matthew L. Kutys, Jinling Yang, Jeroen Eyckmans, Yinyu Wu, Hema Vasavada, Karen K. Hirschi & Christopher S. Chen Nature, doi:10.…
A tunable refractive index matching medium for live imaging cells, tissues and model organisms eLife 2017;6:e27240 DOI: 10.7554/eLife.27240 Boothe T, Hilbert L, Heide M, Berninger L, Huttner WB, Zaburdaev V, Vastenhouw NL, Myers EW, Drechs…
Chemogenetics revealed: DREADD occupancy and activation via converted clozapine. Gomez JL, Bonaventura J, Lesniak W, Mathews WB, Sysa-Shah P, Rodriguez LA, Ellis RJ, Richie CT, Harvey BK, Dannals RF, Pomper MG, Bonci A, Michaelides M Scien…
Whole-Body Profiling of Cancer Metastasis with Single-Cell Resolution Kubota SI, Takahashi K, Nishida J, Morishita Y, Ehata S, Tainaka K, Miyazono K, Ueda HR Cell Rep. 2017 Jul 5;20(1):236-250全身透明化によってがんの全身転移を１細胞の解像…
High-Throughput Mapping of Single-Neuron Projections by Sequencing of Barcoded RNA Kebschull JM, Garcia da Silva P, Reid AP, Peikon ID, Albeanu DF, Zador AM. Neuron. 2016 Sep 7;91(5):975-87.一般的な順行性トレーサーは、特定の領域に存在する…
A fluoro-Nissl dye identifies pericytes as distinct vascular mural cells during in vivo brain imaging Eyiyemisi C Damisah, Robert A Hill, Lei Tong, Katie N Murray & Jaime Grutzendler Nature Neuroscience, 2017, AOP, May 15血液脳関門は様々な…
Iterative expansion microscopy Jae-Byum Chang, Fei Chen, Young-Gyu Yoon, Erica E Jung, Hazen Babcock, Jeong Seuk Kang, Shoh Asano, Ho-Jun Suk, Nikita Pak, Paul W Tillberg, Asmamaw T Wassie, Dawen Cai & Edward S Boyden Nature Methods (2017)…
Multiplexed Intact-Tissue Transcriptional Analysis at Cellular Resolution Emily Lauren Sylwestrak, Priyamvada Rajasethupathy, Matthew Arnot Wright, Anna Jaffe, Karl Deisseroth Cell 2016, 164(4), 792-804CLARITYの架橋にはPFAとアクリルアミド…
A brain microvasculature endothelial cell-specific viral vector with the potential to treat neurovascular and neurological diseases Körbelin J, Dogbevia G, Michelfelder S, Ridder DA, Hunger A, Wenzel J, Seismann H, Lampe M, Bannach J, Pasp…
A Designer AAV Variant Permits Efficient Retrograde Access to Projection Neurons D. Gowanlock R. Tervo, Bum-Yeol Hwang, Sarada Viswanathan, Thomas Gaj, Maria Lavzin, Kimberly D. Ritola,Sarah Lindo, Susan Michael, Elena Kuleshova, David Oja…
Reconstrucion and Simulation of Neocortical Microcircuitry Henry Markram, Eilif Muller, Srikanth Ramaswamy, Michael W. Reimann, …., Javier DeFelipe, Sean L. Hill, Idan Segev, Felix Schürmann et al.,*1 Cell (2015), 163(2), 456–492 何を以て…
ScaleS: an optical clearing palette for biological imaging Hiroshi Hama, Hiroyuki Hioki, Kana Namiki, Tetsushi Hoshida, Hiroshi Kurokawa, Fumiyoshi Ishidate, Takeshi Kaneko, Takumi Akagi, Takashi Saito, Takaomi Saido & Atsushi Miyawaki Nat…
Labelling and optical erasure of synaptic memory traces in the motor cortex Akiko Hayashi-Takagi, Sho Yagishita, Mayumi Nakamura, Fukutoshi Shirai, Yi I. Wu, Amanda L. Loshbaugh, Brian Kuhlman, Klaus M. Hahn, Haruo Kasai Nature (2015), AOP…
Conversion of Channelrhodopsin into a Light-Gated Chloride ChannelJonas Wietek, J. Simon Wiegert, Nona Adeishvili, Franziska Schneider, Hiroshi Watanabe, Satoshi P. Tsunoda, Arend Vogt,Marcus Elstner, Thomas G. Oertner, Peter HegemannScien…
Multiplex Genome Engineering Using CRISPR/Cas SystemsLe Cong, F. Ann Ran, David Cox, Shuailiang Lin, Robert Barretto, Naomi Habib, Patrick D. Hsu, Xuebing Wu, Wenyan Jiang, Luciano A. Marraffini, Feng ZhangScience 339, 819-823 (2013)少し古…
【出典】Optical control of mammalian endogenous transcription and epigenetic statesSilvana Konermann, Mark D. Brigham, Alexandro Trevino, Patrick D. Hsu, Matthias Heidenreich, Le Cong, Randall J. Platt, David A. Scott, George M. Church & F…
【出典】Ultrasensitive fluorescent proteins for imaging neuronal activityTsai-Wen Chen, Trevor J.Wardill, Yi Sun, Stefan R. Pulver, Sabine L. Renninger, Amy Baohan, Eric R. Schreiter, Rex A. Kerr, Michael B. Orger, Vivek Jayaraman, Loren L…
